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RPMI 1640 Medium w/MOPS Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652845

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and… More details

$355 / 10 L +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RPMI 1640 Medium w/MOPS, Culture Media
Full Product Name
RPMI 1640 Medium w/MOPS (Powder)
Form/Format
Light orange , homogenous, free flowing powder
Purity/Purification
Cell Culture Grade
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Endotoxin Level
< 1 EU/ml
pH
5.9
Solubility
Yellow, clear, complete
Directions per Liter
Dissolve 45g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring to final volume. Filter-sterilize using a 0.22um filter. Aliquot into sterile containers.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Components shown as mg/liter
Inorganic Salts
Calcium Nitrate•4H2O 100
Magnesium Sulfate 48.84
Potassium Chloride 400
Sodium Chloride 6000
Sodium Phosphate Dibasic 800
Amino Acids

L-Arginine•HCl 241.86
L-Asparagine•H2O 60
L-Aspartic Acid 20
L-Cystine•2HCl 65.9
L-Glutamic Acid 20
L-Glutamine 300
Glycine 10
L-Histidine•HCl•H2O 20.27
Hydroxy-L-proline 20
L-Isoleucine 50
L-Leucine 50
L-Lysine•HCl 40
L-Methionine 15
L-Phenylalanine 15
L-Proline 20
L-Serine 30
L-Threonine 20
L-Tryptophan 5
L-Tyrosine•Na2•2H2O 28.83
L-Valine 20
Vitamins

p-Aminobenzoic Acid 1
D-Biotin 0.2
Choline Chloride 3
Cyanocobalamin 0.005
Folic Acid 1
myo-Inositol 35
Niacinamide 1
D-Pantothenic Acid, Ca 0.25
Pyridoxine•HCl 1
Riboflavin 0.2
Thiamine•HCl 1
Other
D-Glucose 2000
Glutathione 1
MOPS 34530
Phenol Red, Sodium 5.1

Total: 45 g/liters

Product Categories/Family for RPMI 1640 Medium w/MOPS culture media

Culture Media; Media-Cell Culture; RPMI

Other Notes

Small volumes of RPMI 1640 Medium w/MOPS culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPMI 1640 Medium w/MOPS purchase

MBS652845 is a culture media targeting RPMI 1640 Medium w/MOPS. Also known as RPMI 1640 Medium w/MOPS (Powder).
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