Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine, Cysteine Culture Media

Cell Culture Grade

Ham’s Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Ham’s F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Prepared media should be kept at 4°C and used within a short period of time.

Light pink, homogenous, free flowing powder

Magenta, clear,complete

8.5

< 1 EU/ml

Dissolve 10.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 1.18 g/L sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unti below the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Components shown as g/L
Inorganic Salts:

Calcium Chloride•2H2O Absent
Cupric Chloride•2H2O 0.0000025
Ferrous Chloride 0.000834
Magnesium Chloride•6H2O 0.123
Potassium Chloride 0.224
Sodium Chloride 7.599
Sodium Phosphate Dibasic 0.14204
Zinc Sulfate . 7H2O 0.000863br>
Amino Acids:
L-Alanine 0.009
L-Arginine•HCl 0.211
L-Asparagine•H2O 0.01501
L-Aspartic Acid 0.0133
L-Cysteine•HCL•H2O Absent
L-Glutamic Acid 0.0147
L-Glutamine 0.146
Glycine 0.00751
L-Histidine•HCl•H2O 0.02096
L-Isoleucine 0.00394
L-Leucine 0.0131
L-Lysine•HCl 0.0365
L-Methionin 0.00448
L-Phenylalanine 0.00496
L-Proline 0.0345
L-Serine 0.0105
L-Threonine 0.0119
L-Tryptophan 0.00204
L-Tyrosine•Na2•2H2O 0.00778
L-Valine 0.0117

Vitamins
D-Biotin 0.0000073
Choline Chloride 0.01396
Vitamin B-12 0.00136
Folic Acid 0.00132
myo-Inositol 0.018
Niacinamide 0.000037
D-Pantothenic Acid•Ca 0.00048
Pyridoxine•HCl 0.000062
Riboflavin 0.000038
Thiamine•HCl 0.00034

Other:
Thymidine 0.00073
D-Glucose 1.802
Hypoxanthine 0.00408
Linoleic Acid 0.000084
Thioctic Acid 0.00021
Phenol Red, Sodium 0.0013
Putrescine•2HCl 0.000161
Pyruvic Acid, Sodium 0.11

Total: 10.658 g/liter

Culture Media; Media-Cell Culture; Nutrient Mixture Ham's