RPMI 1640 Medium Modified w/o L-Glutamine, Glucose Culture Media

Pale pink, homogeneous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/ml

Dissolve 8.14g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

8.8

Pink, clear, complete

Inorganic Salts
Calcium Nitrate•4H2O 100
Magnesium Sulfate Anhy 48.8
Potassium Chloride 400
Sodium Chloride 6000
Sodium Phosphate Dibasic 800.7

Amino Acids
L-Arginine HCl 241
L-Asparagine•H2O 56.82
L-Aspartic Acid 20
L-Cystine•2HCl 65.2
L-Glutamic Acid 20
L-Glutamine Absent
Glycine 10
L-Histidine•HCl•H2O 20.2
Hydroxy-L-proline 20
L-Isoleucine 50
L-Leucine 50
L-Lysine•HCl 40
L-Methionine 15
L-Phenylalanine 15
L-Proline 20
L-Serine 30
L-Threonine 20
L-Tryptophan 5
L-Tyrosine•HCl 24
L-Valine 20

Vitamins
p-Amino Benzoic Acid 1
D-Biotin 0.2
Choline Chloride 3
Vitamin B-12 0.005
Folic Acid 1
i-Inositol 35
Niacinamide 1
D-Pantothenic Acid•Ca 0.25
Pyridoxine•HCl 1
Riboflavin 0.2
Thiamine•HCl 1

Other
Glucose Absent
Glutathione 1
Phenol Red, Sodium 5

Total: 8.14g/L

Culture Media; Media-Cell Culture; RPMI