Endothelial Basal Medium MCDB 107 Culture Media

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Light orange, homogenous, free flowing powder

Yellow, clear, complete

5.8

<1EU/ml

Based powder without supplements.

Dissolve 16.2g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts
Calcium Chloride•2H2O: 111
Cupric Sulfate•5H2O: 0.0002497
Ferric Nitrate•9H2O: 1.39
Potassium Chloride: 149
Potassium phosphate mono: 408
Magnesium Sulfate: 120.38
Manganese Sulfate•5H2O: 0.00241
Sodium Chloride: 6599.2
Sodium Selenite•5H2O: 0.00789
Sodium Metasilicate•9H2O: 0.1421
Ammonium Molybdate•4H2O: 0.0012359
Ammonium Metavandate: 0.000585
Nickel Chloride•6H2O: 0.00011885
Stannous Chloride•2H2O: 0.0001128
Zinc Sulfate•7H2O: 0.14375

Amino Acids
L-Alanine: 8.9
L-Arginine: 174.2
L-Asparagine: 13.21
L-Aspartic Acid: 13.31
L-Cysteine: 6.11
L-Glutamic Acid: 14.71
L-Glutamine: 365
Glycine: 22.53
L-Histidine: 15.52
L-Isoleucine: 3.94
L-Leucine: 13.12
L-Lysine: 29.24
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 34.53
L-Serine: 10.51
L-Threonine: 11.91
L-Tryptophan: 2.042
L-Tyrosine: 5.436
L-Valine: 11.72

Vitamins
Biotin: 0.007329
D-Pantothenate, Ca: 0.2383
Choline Chloride: 13.96
Folinic Acid, Ca: 0.0006016
Myo-Inositol: 18.02
Nicotinamide: 6.105
Pyridoxine•HCl: 0.02056
Riboflavin: 0.11292
Thiamine•HCl: 0.3373
Vitamin B12: 0.13554

Other
Adenine: 1.35
D-glucose: 720.8
HEPES Buffer: 7149
Lipoic Acid: 0.002063
Phenol Red, Na: 1.129
Putrescine•2HCI: 0.0001611
Sodium Pyruvate: 110
Thymidine: 0.07266

Total: 16.2g/liter

Culture Media; Media-Cell Culture; MCDB