MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red Culture Media

White, free flowing, homogeneous powder

Store powdered media at Room Temperature. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Colorless, clear, complete

6.2

<1EU/ml

Dissolve 9.27g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 2.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride•2H2O: 264.90
Magnesium Sulfate: 97.70
Potassium Chloride: 400.00
Sodium Chloride: 6800.00
Sodium Phosphate
Monobasic•H2O: 140.30

Amino Acids:
L-Arginine•HCl: 126.40
L-Cystine: 24.02
L-Glutamine: Absent
L-Histidine•HCl•H2O: 42
L-Isoleucine: 52
L-Leucine: 52
L-Lysine•HCl: 72.5
L-Methionine: 15
L-Phenylalanine: 32
L-Threonine: 48
L-Tryptophan: 10
L-Tyrosine: 36
L-Valine: 46

Vitamins:
Choline Chloride: 1.00
Folic Acid: Absent
i-Inositol: 2.00
Nicotinamide: 1.00
D-Pantothenic Acid•Ca: 1.00
Pyridoxal•HCl: 1.00
Riboflavin: 0.10
Thiamine•HCl: 1.00

Other:
D-Glucose: 1000.00
Phenol Red, Sodium: Absent

Total: 9.266g/L

Culture Media; Media-Cell Culture; MEM Eagle