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Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652933

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. More details

$320 / 100 g +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base, Culture Media
Full Product Name
Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base (Powder)
Form/Format
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil:
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Amino Acids
Alanine: 2.0g
p-Aminobenzoic acid: 0.2g
Arginine: 2.0g
Asparagine: 2.0g
Aspartic acid: 2.0g
Cysteine: Absent
Glutamic acid: 2.0g
Glutamine: 2.0g
Glycine: 2.0g
Histidine: Absent
Isoleucine: 2.0g
Leucine: Absent
Lysine: Absent
Methionine: Absent
Phenylalanine: 2.0g
Proline: 2.0g
Serine: 2.0g
Threonine: 2.0g
Tryptophan: 2.0g
Tyrosine: 2.0g
Valine: 2.0g
Nucleotide Bases
Adenine: 2.0g
Uracil: Absent
Vitamins
Inositol: 2.0g

Use 1.3g/liter
100g makes ~50 liters
Appearance
White, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
pH
4.5
Directions per Liter (Broth)
Mix 1.3 grams of drop-out mix with 6.7g MBS653287 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.
Directions per Liter (Plates)
Mix 1.3 grams of drop-out mix with 43.7g MBS653105 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121°C (15psi).

Product Categories/Family for Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base culture media

Culture Media; Media-Yeast

Other Notes

Small volumes of Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base purchase

MBS652933 is a culture media targeting Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base. Also known as Drop-out Mix Synthetic, Minus Methionine, Leucine, Lysine, Histidine, Cysteine, and Uracil, w/o Yeast Nitrogen Base (Powder).
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