RPMI 1640 Medium Modified w/L-Glutamine w/o Glucose, L-Cysteine, Phenol Red Culture Media

Off white, homogenous, free flowing

Cell Culture Grade

Room Temperature

Dissolve 8.32 grams in 900 mL of distilled/deionized (DDI) water, stirring gently until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron membrane. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Inorganic Salts
Calcium Nitrate•4H2O 0.1
Magnesium Sulfate 0.04884
Potassium Chloride 0.4
Sodium Chloride 6
Sodium Phosphate Dibasic 0.8

Amino Acids
L-Arginine 0.2
L-Asparagine 0.05
L-Aspartic Acid 0.02
L-Cystine•2HCl Absent
L-Glutamic Acid 0.02
L-Glutamine 0.3
Glycine 0.01
L-Histidine 0.015
Hydroxy-L-proline 0.02
L-Isoleucine 0.05
L-Leucine 0.05
L-Lysine•HCl 0.04
L-Methionine 0.015
L-Phenylalanine 0.015
L-Proline 0.02
L-Serine 0.03
L-Threonine 0.02
L-Tryptophan 0.005
L-Tyrosine•2Na•2H2O 0.02883
L-Valine 0.02

Vitamins
p-Amino Benzoic Acid 0.001
D-Biotin 0.0002
Choline Chloride 0.003
Vitamin B-12 0.000005
Folic Acid 0.001
myo-Inositol 0.035
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.00025
Pyridoxine•HCl 0.001
Riboflavin 0.0002
Thiamine•HCl 0.001

Other
Glucose Absent
Glutathione 0.001
Phenol Red, Sodium Absent

10Eu/ml

8.51

Colorless, Clear, complete

Culture Media; Media-Cell Culture; RPMI