MEM w/Earle's Temin's Modification w/L-Glutamine, NaHCO3, antibiotics Culture Media

Cell Culture Grade

Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Light pink, homogenous, free flowing powder.

Red, Clear, Complete

7.5

Dissolve 17.6g in 800-900ml of ddH2O stirring gently until completely solubilized. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride 199.59
Ferric Nitrate 0.1
Magnesium Sulfate 100
Potassium Chloride 400
Sodium Chloride 6080
Sodium Phosphate Monobasic 121.4

Amino Acids
L-Arginine•HCl 253.96
L-Cystine•2Na 51.01
L-Glutamine 584
Glycine 30
L-Histidine•HCl 83.76
L-Isoleucine 105
L-Leucine 105
L-Lysine•HCI 145
L-Methionine 30
L-Phenylalanine 64
L-Serine 42
L-Threonine 94
L-Tryptophan 20
L-Tyrosine•2Na 103.79
L-Valine 96

Vitamins
Choline Chloride 2
Folic Acid 2
i-Inositol 4
Nicotinamide 2
D-Pantothenic Acid•Ca 2
Pyridoxal•HCI 2
Riboflavin 0.2
Thiamine•HCl 2

Antibiotics
Penicillin G•Na (~1600u/mg) 60.1
Streptomycin Sulfate (~750mcg/mg) 104.2

Other
D-Glucose 4500
Phenol Red, Sodium 10
Sodium Bicarbonate 4200B

Total: 17.6g/liter

Culture Media; Media-Cell Culture; MEM