Endothelial Basal Medium MCDB 120 Culture Media

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in the dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

< 1EU/ml

7.9

Pink, clear, complete

Off-White, homogeneous, free flowing powder

Dissolve 10.80g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Hygroscopic. Keep tightly closed and desiccated.

Inorganic Salts:
Ammonium Metavanadate 0.0006
Ammonium molybdate•4H2O 0.00371
Calcium Chloride•2H2O 235.2
Cupric Sulfate•5H2O 0.0025
Ferrous Sulfate•7H2O 0.834
Magnesium Sulfate•anhydrous 120.4
Manganese Sulfate•H20 0.000241
Nickel Chloride•6H2O 0.0000713
Potassium Chloride 298.2
Sodium Chloride 6428.4
Sodium Meta Silicate•9H2O 2.842
Sodium Phosphate Dibasic 71
Zinc Sulfate•7H2O 0.08625

Amino Acids:
L-Alanine 2.67
L-Arginine Hydrochloride 210.67
L-Asparagine•H2O 15.01
L-Aspartic Acid 13.31
L-Cysteine•HCl•H2O 35.12
L-Glutamic Acid 4.41
L-Glutamine 1461.5
Glycine 2.25
L-Histidine Hydrochloride•H2O 41.93
L-Isoleucine 65.58
L-Leucine 131.2
L-Lysine Hydrochloride 181.65
L-Methionine 29.84
L-Phenylalanine 33.04
L-Proline 11.51
L-Serine 31.53
L-Threonine 35.73
L-Tryptophan 4.08
L-Tyrosine•2Na•2H2O 22.52
L-Valine 117.15

Vitamins:
Biotin 0.00733
Choline Chloride 13.96
Vitamin B12 0.0136
Folinic Acid Calcium 0.5115
i-Inositol 10.09
Nicotinamide 6.105
D-Pantothenic Acid (hemi Ca) 47.7
Pyridoxine Hydrochloride 2.056
Riboflavin 0.0038
Thiamine Hydrochloride 3.373

Other:
Adenine HCl 0.1716
Thymidine 0.0242
D-Glucose 1000
Lipoic Acid 0.0021
Phenol Red 1.2421
Putrescine•2HCl 0.0001611
Sodium Pyruvate 110
Sodium Selenite 0.0052

Total: 10.80g/L

Culture Media; Media-Cell Culture; MCDB