L-Broth Culture Media

L-Broth (Luria Broth) is the standard nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, plasmid propagation, and protein expression. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950's (1,2,3). These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin.

Small volumes of L-Broth culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS652981 is a culture media targeting L-Broth. Also known as L-Broth (Luria Broth) (Powder).