Dulbecco's MEM Culture Media

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/ml

White homogeneous, free-flowing powder.

Colorless, clear, complete.

7.2

Dissolve 10g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Components shown as: g/Liter

Inorganic Salts:
Calcium Chloride•2H2O: 0.265
Ferric Nitrate•9H2O: 0.0001
Magnesium Sulfate: 0.09767
Potassium Chloride: 0.4
Sodium Chloride: 6.4
Sodium Phosphate Monobasic: 0.109

Amino Acids:
L-Arginine•HCl: 0.084
L-Cystine•2HCl: 0.0626
L-Glutamine: 0.584
Glycine: 0.03
L-Histidine•HCl•H2O: 0.042
L-Isoleucine: 0.105
L-Leucine: 0.105
L-Lysine•HCl: 0.146
L-Methionine: 0.03
L-Phenylalanine: 0.066
L-Serine: 0.042
L-Threonine: 0.095
L-Tryptophan: 0.016
L-Tyrosine•2Na•2H2O: 0.10379
L-Valine: 0.094

Vitamins:
Choline Chloride: 0.004
Folic Acid: Absent
myo-Inositol: 0.0072
Niacinamide: Absent
D-Pantothenic Acid•Ca: 0.004
Pyridoxal•HCl: Absent
Riboflavin: Absent
Thiamine•HCl: Absent

Other
D-Glucose: 1
Phenol Red, Sodium: Absent
Pyruvic Acid, Sodium: 0.11

Total: 10g/L