RPMI 1640 Medium w/o L-Glutamine, Inositol Culture Media

Light pink, homogenous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degrees C and used within a short period of time.

<1EU/mL

Pink, clear, complete

8.9

Dissolve 10.1g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Components shown as mg/L:
Inorganic Salts:
Calcium Nitrate•4H2O: 100
Magnesium Sulfate: 48.84
Potassium Chloride: 400
Sodium Chloride: 6000
Sodium Phosphate Dibasic: 800

Amino Acids:
L-Arginine: 200
L-Asparagine•H2O: 50
L-Aspartic Acid: 20
L-Cystine•2HCl: 65.2
L-Glutamic Acid: 20
Glycine: 10
L-Histidine: 15
Hydroxy-L-proline: 20
L-Isoleucine: 50
L-Leucine: 50
L-Lysine•HCl; 40
L-Methionine: 15
L-Phenylalanine: 15
L-Proline: 20
L-Serine: 30
L-Threonine: 20
L-Tryptophan: 5
L-Tyrosine•2Na•2H2O: 28.83
L-Valine: 20

Vitamins:
P-Amino Benzoic Acid: 1
D-Biotin: 0.2
Choline Chloride: 3
Vitamin B-12: 0.005
Folic Acid: 1
Nicotinamide: 1
D-Pantothenic Acid•Ca: 0.25
Pyridoxine•HCl: 1
Riboflavin: 0.2
Thiamine•HCl: 1

Other:
D-Glucose: 2000
Glutathione: 1
Phenol Red, Sodium: 5

Total: 10.1g/L

Culture Media; Media-Cell Culture; RPMI