RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin Culture Media

Light pink, homogenous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degrees C and used within a short period of time.

<=1EU/ml

8.6

Pink. clear, complete

Dissolve 10.092g in 900ml of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2.0g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Components shown as g/L

Inorganic Salts
Calcium Nitrate*4H2O: 0.100
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.400
Sodium Chloride: 6.000
Sodium Phosphate Dibasic: 0.800

Amino: Acids
L-Alanine: Absent
L-Arginine: 0.200
L-Asparagine: 0.050
L-Aspartic Acid: 0.020
L-Cystine*2HCl: 0.065
L-Glutamic Acid: 0.020
L-Glutamine: Absent
Glycine: 0.010
L-Histidine: 0.015
Hydroxy-L-proline: 0.020
L-Isoleucine: 0.050
L-Leucine: 0.050
L-Lysine*HCl: 0.040
L-Methionine: 0.015
L-Phenylalanine: 0.015
L-Proline: 0.020
L-Serine: 0.030
L-Threonine: 0.020
L-Tryptophan: 0.005
L-Tyrosine*HCl: 0.028
L-Valine: 0.020

Vitamins
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin (Vit.B12): 0.000005
Folic Acid: Absent
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid, Ca: 0.00025
Pyridoxal*HCl: 0.001
Riboflavin: Absent
Thiamine*HCl: 0.001

Other
D-Glucose: 2.000
Glutathione, reduced: 0.001
Phenol Red, Sodium: 0.0053

Total: 10.092g/L

Culture Media; Media-Cell Culture; RPMI