Dulbecco's MEM Culture Media

Light orange, homogeneous, free flowing powder.

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

< 1 EU/ml

7.8

Pink, clear, complete

Dissolve 12.048g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not auto clave. Contains heat-labile compounds that can be damaged with auto claving.

Inorganic Salts:
Calcium Chloride 2H2O: 0.154
Cupric Sulfate•H2O: 0.0000013
Ferric Nitrate•9H2O: 0.00005
Ferrous Sulfate•7H2O: 0.000417
Magnesium Chloride 6H2O: 0.0612
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.3118
Sodium Chloride: 6.996
Sodium Phosphate dibasic: 0.07102
Sodium Phosphate monobasic: 0.0543
Zinc Sulfate•7H2O: 0.000432

Amino Acids:
L-Alanine: 0.00445
L-Arginine•HCl: 0.1475
L-Asparagine•H2O: 0.0075
L-Aspartic Acid: 0.00665
L-Cystine•2HCl: 0.03129
L-Cysteine•HCl•H2O: 0.01756
L-Glutamic Acid : 0.00735
L-Glutamine: 0.365
Glycine: 0.01875
L-Histidine HCl•H2O: 0.03148
L-Isoleucine: 0.05447
L-Leucine: 0.05905L
L-Lysine•HCl: 0.09125
L-Methionine: 0.01724
L-Phenylalanine: 0.03548
L-Proline: 0.01725
L-Serine: 0.02625
L-Threonine: 0.05345
L-Tryptophan: Absent
L-Tyrosine•2Na•2H20: 0.05579
L-Valine: 0.05285

Vitamins:
Biotin: 0.0000035
Choline Chloride: 0.00898
Folic Acid: 0.00266
i-inositol: 0.0126
Nicotinamide: 0.00202
D-Pantothenic Acid•Ca: 0.00224
Pyridoxine HCl: 0.000031
Pyridoxal Hydrochloride: 0.002
Riboflavin: 0.000219
Thiamine HCl: 0.00217
Vitamin B12: 0.00068

Other:
D-Glucose: 3.15
Hypoxanthine(Na): 0.0021
Linoleic Acid: 0.000042
Lipoic Acid: 0.000105
Phenol Red: 0.0081
Putrescine•HCl: 0.000081
Pyruvic Acid, Sodium: 0.055
Thymidine: 0.000365

Total: 12.048g/Liter

Culture Media; Media-Cell Culture; DMEM