Russell Double Sugar Agar Culture Media

Russell Double Sugar Agar is a differential agar medium used for the identification of enteric bacteria based on the fermentation of dextrose and lactose with or without gas production.

The original formula of Russell Double Sugar Agar developed by Russell was modified by Nicols by replacing litmus indicator with phenol red. Selected colonies are transferred from the primary plating medium to tubes of Russell Double Sugar Agar using a straight bacteriological wire, stabbing the butt of the tube and streaking the slanted portion of the medium. Inoculated tubes after incubation are observed for pH reactions and gas formation. Phenol red indicates fermentation of the carbohydrates by the production of a yellow color (acid reaction); lack of fermentation is indicated by a red color (alkaline reaction). Gas formation is indicated by the presence of bubbles or cracks in the medium. After 24-48 hours incubation under aerobic conditions, a tube with a red slant and yellow butt indicates dextrose fermentation. Lactose fermentation is indicated by a yellow slant and butt. A red slant and butt indicates no fermentation. Other biochemical or serological tests are performed as required to confirm identification.

Small volumes of Russell Double Sugar Agar culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS653062 is a culture media targeting Russell Double Sugar Agar. Also known as Russell Double Sugar Agar (Powder).