Williams' Medium E w/L-Glutamine w/o Glucose Culture Media

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Note: Hygroscopic. Keep tightly closed and dessicated.

Light orange, homogeneous, free flowing powder

< 1EU/ml

4.4

Yellow, clear, complete

Dissolve 8.8g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Components shown as g/liter

Inorganic Salts
Calcium Chloride•2H2O 0.265
Cupric Sulfate•H2O 0.0000001
Ferric Nitrate•9H2O 0.0000001
Magnesium Sulfate 0.0977
Manganese Chloride•4H2O 0.0000001
Potassium Chloride 0.4
Sodium Chloride 6.8
Sodium Phosphate Monobasic 0.122
Zinc Sulfate•7H2O 0.0000002

Amino Acids
L-Alanine 0.09
L-Arginine 0.05
L-Asparagine•H2O 0.02
L-Aspartic Acid 0.03
L-Cysteine (free acid) 0.04
L-Cystine*2HCl 0.02
L-Glutamic Acid 0.0445
L-Glutamine 0.292
Glycine 0.05
L-Histidine 0.015
L-Isoleucine 0.05
L-Leucine 0.075
L-Lysine•HCl 0.08746
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.03
L-Serine 0.01
L-Threonine 0.04
L-Tryptophan 0.01
L-Tyrosine•2Na•2H2O 0.05045
L-Valine 0.05

Vitamins
Ascorbic Acid•Na 0.00227
D-Biotin 0.0005
Choline Chloride 0.0015
Vitamin B-12 0.0002
Calciferol 0.0001
Folic Acid 0.001
myo-Inositol 0.002
Menadione (sodium bisulfate) 0.00001
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.001
Pyridoxal•HCl 0.001
Riboflavin 0.0001
Thiamine•HCl 0.001
DL-a-Tocopherol Phosphate•2Na 0.00001
Retinol Acetate 0.0001

Other
D-Glucose Absent
Glutathione 0.00005
Methyl Linoleate 0.00003
Phenol Red, Sodium 0.0107
Pyruvic Acid, Sodium 0.025

Total: 8.8g/L

Culture Media; Media-Cell Culture; Williams'