Cell Attachment Reagent Culture Media

Cell Culture Grade

Store at 4°C. Do Not Freeze. Stable for 6 months.

Lot analysis: Pass

7.3

18.3 mS

(0.1mL into each of the following media, in triplicate; 37°C/5-7 days.)
Nutrient Broth: Tube # 1: Negative; Tube #2: Negative; Tube #3: Negative
Thioglycollate Medium: Tube #1: Negative; Tube #2: Negative; Tube #3: Negative

Specification: >50% cell attachment to coated plastic substratum vs. uncoated substratum; Lot analysis : Pass

Color is light to medium pink due to presence of pH indicator. Variations in color has no effect upon performance of product. Color fades with age.

To coat the growth surface, add 0.2ml/cm2 of surface area of the culture vessel. For example, use 5ml for a T-25 flask. The surface should be completely covered with a layer of liquid. Incubate for 30 seconds. Remove the Cell Attachment Reagent with a pipet. Residual Cell Attachment Reagent will not adversely affect the viability of the cells to be plated. Do not return any “used” reagent to the original container. This will compromise the performance of the product. The coating may be performed immediately or up to one hour prior to seeding the cells.

Culture Media; Media-Cell Culture