Endothelial Basal Medium MCDB 131 w/o Phenol Red, Cysteine, Methionine Culture Media

White , homogenous, free-flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/ml

7.6

Colorless, clear, complete

Dissolve 11.48g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Ammonium Metavanadate 0.0006
Ammonium Molybdate•4H2O 0.0037
Calcium Chloride•2H2O 235
Cupric Sulfate•5H2O 0.0012
Ferrous Sulfate•7H2O 0.283
Magnesium Sulfate•7H2O 2464
Manganese Sulfate•H20 0.0002
Nickel Chloride•6H2O 7.1
Potassium Chloride 298
Sodium Chloride 6430
Sodium Meta Silicate•9H2O 2.8
Sodium Phosphate Dibasic•7H2O 134
Zinc Sulfate•H2O 0.0003

Amino Acids:
L-Alanine 2.7
L-Arginine Hydrochloride 63.2
L-Asparagine•H2O 15
L-Aspartic Acid 13.3
L-Cysteine•2HCl•H2O absent
L-Glutamic Acid 4.4
Glycine 2.3
L-Histidine Hydrochloride•H2O 42
L-Isoleucine 66
L-Leucine 131
L-Lysine Hydrochloride 182
L-Methionine absent
L-Phenylalanine 33
L-Proline 11.5
L-Serine 32
L-Threonine 12
L-Tryptophan 4.1
L-Tyrosine 18.1
L-Valine 117

Vitamins:
Biotin 0.0073
Choline Chloride 14
Vitamin B12 0.0136
Folinic Acid Calcium 0.6
i-Inositol 7.2
Niacinamide 6.1
D-Calcium Pantothenate 12
Pyridoxine Hydrochloride 2.1
Riboflavin 0.0038
Thiamine Hydrochloride 3.4

Other Components:
Adenine 0.135
Thymidine 0.024
D-Glucose1000
Lipoic Acid 0.0021
Phenol Red absent
Putrescine 2HCI 0.0002
Selenious Acid 0.0038
Sodium Pyruvate 110

Total: 11.48g/L

Culture Media; Media-Cell Culture; MCDB