CMRL1066 w/o L-Glutamine, Glucose Culture Media

Appereance: Orange, homogenous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Yellow, clear, complete

4.0

< 1 EU/ml

Dissolve 8.73g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts
Calcium Chloride, anhy 0.2
Magnesium Sulfate, anhy 0.09769
Potassium Chloride 0.4
Sodium Acetate, anhy 0.05
Sodium Chloride 6.8
Sodium Phosphate Monobasic, anhy 0.122

Amino Acids
L-Alanine 0.025
L-Arginine 0.05787
L-Aspartic Acid 0.03
L-Cysteine•HCL•H2O 0.26
L-Cystine 0.02
L-Glutamic Acid, Sodium 0.0862
L-Glutamine Absent
Glycine 0.05
L-Histidine•HCl•H2O 0.02
trans-4-Hydroxy-L-Proline 0.01
L-Isoleucine 0.02
L-Leucine 0.06
L-Lysine•HCl 0.07
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.04
L-Serine 0.025
L-Threonine 0.03
L-Tryptophan 0.01
L-Tyrosine 0.04
L-Valine 0.025

Vitamins:
PABA 0.00005
L-Ascorbic Acid•Na 0.05
D-Biotin 0.00001
Choline Chloride 0.0005
Cocarboxylase 0.001
Coenzyme A•Na 0.0025
2'-Deoxyadenosine 0.01
2'-Deoxycytidine•HCl 0.0116
2'-Deoxyguanosine 0.01
Folic Acid 0.00001
myo-Inositol 0.00005
5-Methyldeoxycytidine 0.0001
B-NAD 0.007
B-NADP•Na 0.001
Nicotinic Acid 0.000025
Niacinamide 0.000025
D-PantothenicAcid•Ca 0.00001
Pyridoxal•HCl 0.000025
Pyridoxine•HCl 0.000025
Riboflavin 0.00001
Flavin Adenine Dinucleotide•2Na 0.000106
Thiamine•HCl 0.00001

Other
Cholesterol 0.0002
Thymidine 0.01
D-Glucose Absent
D-GlucuronicAcid•Na 0.00388
Glutathione 0.01
Phenol Red ,Sodium 0.02124
Tween 80 0.005
Uridine-5-Triphosphate•4Na 0.00113

Total: 8.73g/liter

Culture Media; Media-Cell Culture