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RPMI 1640 Medium w/L-Glutamine Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653142

RPMI-1640 was developed by Moore, et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and… More details

$355 / 10 L +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RPMI 1640 Medium w/L-Glutamine, Culture Media
Full Product Name
RPMI 1640 Medium w/L-Glutamine (Powder)
Purity/Purification
Cell Culture Grade
Preparation and Storage
RT/4 degree C
Endotoxin Level
<1EU/ml
Appearance
Orange, homogenous, free flowing powder
pH
8.6
Solubility
Magenta, clear, complete
Direction Per Liter
Dissolve 10.4g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.
Note
It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Inorganic Salts
Calcium Nitrate 4H2O 0.1
Magnesium Sulfate 0.04884
Potassium Chloride 0.4
Sodium Chloride 6.0
Sodium Phosphate Dibasic 0.8
Amino Acids
L-Arginine 0.2
L-Aspartic Acid 0.02
L-Asparagine 0.05
L-Cystine•2HCl 0.0652
L-Glutamic Acid 0.02
L-Glutamine 0.3
Glycine 0.01
L-Histidine 0.015
Hydroxy-L-proline 0.02
L-Isoleucine 0.05
L-Leucine 0.05
L-Lysine•HCl 0.04
L-Methionine 0.015
L-Phenylalanine 0.015
L-Proline 0.02
L-Serine 0.03
L-Threonine 0.02
L-Tryptophan 0.005
L-Tyrosine•2Na•2H2O 0.02883
L-Valine 0.02.
Vitamins
p-Aminobenzoic Acid 0.001
D-Biotin 0.0002
Choline Chloride 0.003
Cyanocobalamin 0.000005
Folic Acid 0.001
myo-Inositol 0.035
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.00025
Pyridoxine•HCl 0.001
Riboflavin 0.0002
Thiamine•HCl 0.001
Other
D-Glucose 2.0
Glutathione (reduced) 0.001
Phenol Red, sodium 0.0053
Total
10.4g/liter

Product Categories/Family for RPMI 1640 Medium w/L-Glutamine culture media

Culture Media; Media-Cell Culture; RPMI

Other Notes

Small volumes of RPMI 1640 Medium w/L-Glutamine culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPMI 1640 Medium w/L-Glutamine purchase

MBS653142 is a culture media targeting RPMI 1640 Medium w/L-Glutamine. Also known as RPMI 1640 Medium w/L-Glutamine (Powder).
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