Ames Medium w/L-Glutamine Culture Media

Cell Culture Grade

Store powdered media at 4°C. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/ml

White, homogenous, free flowing powder

Colorless, clear, complete

4.5

Gas ~900ml of water (15-20°C) with 100% CO2. While gently stirring the water, add 1.9g sodium bicarbonate. Add 8.8g of the powder medium and stir until dissolved. Adjust the pH of the medium to 0.2-0.3 pH unit below the desired level using 1N NaOH and 1N HCl. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts
Calcium Chloride - 0.1275
Magnesium Sulfate - 0.1488
Potassium Chloride - 0.231
Potassium Phosphate Mono. - 0.068
Sodium Chloride - 7.01

Amino Acids
L-Alanine - 0.0024
L-Arginine•HCl - 0.00421
L-Aspartic Acid - 0.00012
L-Asparagine - 0.00084
L-Cystine•2HCl - 0.000065
L-Glutamic Acid, Sodium - 0.001183
L-Glutamine - 0.073
Glycine - 0.00045
L-Histidine•HCl•H2O - 0.002513
L-Isoleucine - 0.00058
L-Leucine - 0.00144
L-Lysine•HCl - 0.003648
L-Methionine - 0.00039
L-Phenylalanine - 0.00132
L-Proline - 0.00007
L-Serine - 0.00252
Taurine - 0.00075
L-Threonine - 0.00333
L-Tryptophan - 0.00049
L-Tyrosine•2Na•2H2O - 0.00211
L-Valine - 0.00176

Vitamins
Ascorbic Acid•Na - 0.01796
D-Biotin - 0.0001
Choline Chloride - 0.0007
Folic Acid - 0.0001
myo-Inositol - 0.0272
Niacinamide - 0.0001
D-Pantothenic Acid•Ca - 0.0001
Pyridoxal•HCl - 0.0001
Riboflavin - 0.00001
Thiamine•HCl - 0.0001

Other
Cytidine - 0.00073
Thymidine - 0.00024
D-Glucose - 1.081
Hypoxanthine - 0.00082
Pyruvic Acid, Sodium - 0.01333
Uridine - 0.00073

Total: 8.8 g/liter

Culture Media; Media-Cell Culture