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Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653192

MCDB 131 was developed by Knedler and Ham as a reduced serum-supplemented medium for the culture of human microvascular endothelial cells. This formulation has been reported to support clonal growth of human omental microvascular cells when supplemented with… More details

$390 / 10 L +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red, Culture Media
Full Product Name
Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red (Powder)
Form/Format
Pale orange, homogenous, free-flowing powder
Purity/Purification
Cell Culture Grade
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Solubility
Pink, clear, complete
pH
7.4
Endotoxin
< 1 EU/ml
Directions per Liter
Dissolve 11.52g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as mg/L
Inorganic Salts
Ammonium Metavanadate 0.0006
Ammonium Molybdate•4H2O 0.0037
Calcium Chloride•2H2O 235
Cupric Sulfate•5H2O 0.0012
Ferrous Sulfate•7H2O 0.283
Magnesium Sulfate•7H2O 2464
Manganese Sulfate•H20 0.0002
Nickel Chloride•6H2O 0.000071
Potassium Chloride 298
Sodium Chloride 6430
Sodium Meta Silicate•9H2O 2.8
Sodium Phosphate Dibasic•7H2O 134
Zinc Sulfate•H2O 0.0003
Amino Acids

L-Alanine 2.7
L-Arginine Hydrochloride 63.2
L-Asparagine•H2O 15
L-Aspartic Acid 13.3
L-Cysteine•2HCl.•H2O 35
L-Glutamic Acid 4.4
Glycine 2.3
L-Histidine Hydrochloride•H2O 42
L-Isoleucine 66
L-Leucine 131
L-Lysine Hydrochloride 182
L-Methionine 15
L-Phenylalanine 33
L-Proline 11.5
L-Serine 32
L-Threonine 12
L-Tryptophan 4.1
L-Tyrosine 18.1
D-Valine 117
Vitamins

Biotin 0.0073
Choline Chloride 14
Vitamin B12 0.0136
Folinic Acid Calcium 0.6
i-Inositol 7.2
Niacinamide 6.1
D-Calcium Pantothenate 12
Pyridoxine Hydrochloride 2.1
Riboflavin 0.0038
Thiamine Hydrochloride 3.4

Total: 11.52g/L

Product Categories/Family for Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red culture media

Culture Media; Media-Cell Culture; MCDB

Other Notes

Small volumes of Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red purchase

MBS653192 is a culture media targeting Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red. Also known as Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red (Powder).
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