CMRL1066 10X w/o L-Glutamine Culture Media

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Light pink, homogenous , free flowing powder

Orange turbid, incomplete at 10X Yellow , clear, complete at 1X

4.0 (10x)
4.3 (1X)

< 1 EU/mL

Dissolve 97.34g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 22g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Some precipitate may be observed due to excess concentration of salts. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Note: USB recommends the preparation of this product as a 1X solution.

Inorganic Salts:
Calcium Chloride 2.00
Magnesium Sulfate, anhy 0.9769
Potassium Chloride 4.00
Sodium Acetate, anhy 0.50
Sodium Chloride 68.0
Sodium Phosphate Mono, anhy 1.22

Amino Acids:
L-Alanine 0.25
L-Arginine 0.5787
L-Aspartic Acid 0.30
L-Cysteine•HCL•H2O 2.60
L-Cystine 0.20
L-Glutamic Acid, Sodium 0.862
L-Glutamine Absent
Glycine 0.50
L-Histidine•HCl•H2O 0.20
trans-4-Hydroxy-L-Proline 0.10
L-Isoleucine 0.20
L-Leucine 0.60
L-Lysine•HCl 0.70
L-Methionine 0.15

Components shown as g/liter 10X:
L-Phenylalanine 0.25
L-Proline 0.40
L-Serine 0.25
L-Threonine 0.30
L-Tryptophan 0.10
L-Tyrosine 0.40
L-Valine 0.25

Vitamins:
PABA 0.0005
L-Ascorbic Acid•Na 0.50
D-Biotin 0.0001
Choline Chloride 0.005
Coenzyme A•Na 0.025
Cocarboxylase 0.01
2'-Deoxyadenosine 0.10
2'-Deoxycytidine•HCl 0.116
2'-Deoxyguanosine 0.10
Folic Acid 0.0001
myo-Inositol 0.0005
5-Methyldeoxycytidine 0.001
ß-NAD 0.070
ß-NADP•Na 0.010
Nicotinic Acid 0.00025
Niacinamide 0.00025
D-Pantothenic Acid•Ca 0.0001
Pyridoxal•HCl 0.00025
Pyridoxine•HCl 0.00025
Riboflavin 0.0001
Flavin Adenine Dinucleotide•2Na 0.00106
Thiamine•HCl 0.0001

Other:
Cholesterol 0.002
Thymidine 0.10
D-Glucose 10.0
D-Glucuronic Acid•Na 0.0388
Glutathione 0.10
Phenol Red, Sodium 0.2124
Tween80 0.05
Uridine-5-Triphosphate•4Na 0.0113

Total: 97.34 g/Liter

Culture Media; Media-Cell Culture