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Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653231

MBS653231 is a culture media targeting Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base. Also known as Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder). More details

$320 / 100 g +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base, Culture Media
Full Product Name
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder)
Form/Format
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus leucine:
Purity/Purification
Molecular Biology Grade
Specificity
Drop-out Mix Synthetic- minus Leucine (LeuDo) is used as an addition to Drop-out Base for developing synthetic drop-out media formulations for selecting leucine auxotrophic cells.
Preparation and Storage
Store powder media at RT. Open bottles should be capped tightly and kept in the dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Appearance
White, homogenous, free flowing powder
Amino Acids
Alanine 2.0g
p-Aminobenzoic 0.2g
Arginine 2.0g
Asparagine 2.0g
Aspartic acid 2.0g
Cysteine 2.0g
Glutamic acid 2.0g
Glutamine 2.0
Glycine 2.0g
Histidine 2.0g
Isoleucine 2.0g
Leucine Absent
Lysine 2.0g
Methionine 2.0g
Phenylalanine 2.0g
Proline 2.0g
Serine 2.0g
Threonine 2.0g
Tryptophan 2.0g
Tyrosine 2.0g
Valine 2.0g
Nucleotide Bases
Adenine 0.5g
Inositol 2.0g
Uracil 2.0g

Use 1.62g/liter
pH
4.2
Solubility
Colorless, clear, complete
Direction per Liter (Broth)
Mix 1.62 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate &/AS per liter of ddH20. Adjust to desire pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.
Directions per Liter(Plates)
Mix 1.62 grams of drop-out mix with 43.7g D9510-01 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH20. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121°C (15psi).

Product Categories/Family for Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base culture media

Culture Media; Media-Yeast

Other Notes

Small volumes of Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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