RPMI 1640 Medium Deficient w/L-Glutamine, w/o Isoleucine Culture Media

Light orange, homogeneous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.

8.6

Pink, clear, complete

≤ 1EU/ml

Dissolve 10.3g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add 2g/L sodium bicarbonate with stirring. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Inorganic Salts:
Calcium Nitrate - 4H2O: 0.10
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.40
Sodium Chloride: 6.00
Sodium Phosphate Dibasic: 0.80

Amino Acids:
L-Arginine: 0.20
L-Asparagine: 0.05
L-Aspartic Acid: 0.02
L-Cystine-2HCl: 0.0652
L-Glutamic Acid: 0.02
L-Glutamine: 0.30
Glycine: 0.01
L-Histidine: 0.015
Hydroxy-L-proline: 0.02
L-Isoleucine- absent
L-Leucine: 0.05
L-Lysine-HCl: 0.04
L-Methionine: 0.015
L-Phenylalanine: 0.015
L-Proline: 0.02
L-Serine: 0.03
L-Threonine: 0.02
L-Tryptophan: 0.005
L-Tyrosine-2Na-2H2O: 0.02883
L-Valine: 0.02

Vitamins:
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin: 0.000005
Folic Acid: 0.001
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid-Ca: 0.00025
Pyridoxine-HCl: 0.001
Riboflavin: 0.0002
Thiamine-HCl: 0.001

Other:
D-Glucose: 2.0
Glutathione: 0.001
Phenol Red, sodium: 0.0053

Total: 10.3g/Liter

Culture Media; Media-Cell Culture; RPMI