Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS Culture Media

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with “Drop Out” medium supplement mixtures, Y2025 provides an excellent medium to cultivate and select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.

Small volumes of Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS653287 is a culture media targeting Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS. Also known as Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder). Host: Yeast.