RPMI 1640 Medium Deficient w/o L-Glutamine, Methionine, Cystine Culture Media

Pale pink, homogenous, free flowing powder.

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/ml

Pale orange, homogenous, free flowing powder

8.4

Magenta, clear, complete.

Dissolve 10g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Inorganic Salts
Calcium Nitrate•4H2O - 100
Magnesium Sulfate - 48.8
Potassium Chloride - 400
Sodium Chloride - 6000
Sodium Phosphate Dibasic - 800.7

Amino Acids
L-Arginine - 200
L-Asparagine - 50
L-Aspartic Acid - 20
L-Glutamic Acid - 20
Glycine - 10
L-Histidine - 15
Hydroxy-L-proline - 20
L-Isoleucine - 50
L-Leucine - 50
L-Lysine•HCl - 40
L-Phenylalanine - 15
L-Proline - 20
L-Serine - 30
L-Threonine - 20
L-Tryptophan - 5
L-Tyrosine•2Na•2H2O - 28.83
L-Valine - 20

Vitamins
p-Aminobenzoic Acid - 1
D-Biotin - 0.2
Choline Chloride - 3
Cyanocobalamin - 0.005
Folic Acid - 1
i-Inositol - 35
Nicotinamide - 1
D-Pantothenic Acid, Ca - 0.25
Pyridoxine•HCl - 1
Riboflavin - 0.2
Thiamine•HCl - 1

Other
D-Glucose 2000
Glutathione,Reduced 1
Phenol Red, Sodium 5.3

Total: 10g/L

Culture Media; Media-Cell Culture; RPMI