Dulbecco's MEM Culture Media

Light orange, homogenous, free flowing powder

Store powdered media at RT.
Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Prepared media should be kept at 4 degree C and used within a short period of time.

Cell Culture Grade

<1EU/ml

Dissolve 9.77g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

7.1

Orange, Clear, Complete

Inorganic Salts
Ferric Nitrate-9H2O: 0.0001
Magnesium Sulfate: 0.09767
Potassium Chloride: 0.40
Sodium Chloride: 6.40
Sodium Phosphate Monobasic: 0.109

Amino Acids
L-Arginine-HCl: 0.084
L-Cystine-2HCl: 0.0626
L-Glutamine: 0.584
Glycine: 0.030
L-Histidine-HCl-H2O: 0.042
L-Isoleucine: 0.105
L-Leucine: 0.105
L-Lysine-HCl: 0.146
L-Methionine: 0.03
L-Phenylalanine: 0.066
L-Serine: 0.042
L-Threonine: 0.095
L-Tryptophan: 0.016
L-Tyrosine-2Na-2H2O: 0.10379
L-Valine: 0.094

Vitamins
Choline Chloride: 0.004
Folic Acid: 0.004
myo-Inositol: 0.0072
Niacinamide: 0.004
D-Pantothenic Acid, Na: 0.004
Pyridoxal-HCl: 0.004
Riboflavin: 0.0004
Thiamine-HCl: 0.004

Other
D-Glucose: 1.00
Phenol Red, Sodium: 0.0159
Pyruvic Acid, Sodium: 0.11

Total: 9.77g/Liter

Culture Media; Media-Cell Culture; DMEM