CMRL1066 w/L-Glutamine Culture Media

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.

Light Orange, homogeneous, free flowing powder

Yellow, clear, complete

< 1EU/ml

3.9

Dissolve 9.82g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride, 0.2
Magnesium Sulfate, 0.09769
Potassium Chloride 0.4
Sodium Acetate, 0.05
Sodium Chloride 6.8
Sodium Phosphate Monobasic, 0.122

Amino Acids
L-Alanine 0.025
L-Arginine 0.05787
L-Aspartic Acid 0.03
L-Cysteine•HCL•H2O 0.26
L-Cystine•2HCl 0.02
L-Glutamic Acid, Sodium 0.075
L-Glutamine 0.1
Glycine 0.05
L-Histidine•HCl•H2O 0.02
trans-4-Hydroxy-L-Proline 0.01
L-Isoleucine 0.02
L-Leucine 0.06
L-Lysine•HCl 0.07
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.04
L-Serine 0.025
L-Threonine 0.03
L-Tryptophan 0.01
L-Tyrosine 0.04
L-Valine 0.025

Vitamins
PABA 0.00005
L-Ascorbic Acid•Na 0.05
D-Biotin 0.00001
Choline Chloride 0.0005
Coenzyme A•Na 0.0025
Cocarboxylase 0.001
2'-Deoxyadenosine 0.01
2'-Deoxycytidine•HCl 0.0116
2'-Deoxyguanosine 0.01
Folic Acid 0.00001
myo-Inositol 0.00005
5-Methyldeoxycytidine 0.0001
b-NAD 0.007
b-NADP•Na 0.001
Nicotinic Acid 0.000025
Niacinamide 0.000025
D-Pantothenic Acid•Ca 0.00001
Pyridoxal•HCl 0.000025
Pyridoxine•HCl 0.000025
Riboflavin 0.00001
Flavin Adenine Dinucleotide•2Na 0.000106
Thiamine•HCl 0.00001

Other
Cholesterol 0.0002
Thymidine 0.01
D-Glucose 1.00
D-GlucuronicAcid•Na 0.00388
Glutathione 0.01
Phenol Red,Sodium 0.02124
Tween 80 0.005
Uridine-5-Triphosphate•4Na 0.001

Total: 9.823g/liter

Culture Media; Media-Cell Culture