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Drop-out Mix Complete, Adenine Low Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653334

Drop-out Mixes are used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants More details

$320 / 100 g +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Drop-out Mix Complete, Adenine Low, Culture Media
Full Product Name
Drop-out Mix Complete, Adenine Low (0.118g), High Tyrosine (4g) and Phenylalanine (4g) w/o Yeast Nitrogen Base (Powder)
Form/Format
White, homogeneous, free flowing powder
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time
Solubility
Colorless, clear, complete
pH
4.2
Directions per Liter (Broth)
Mix 2.14 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.
Directions per Liter (Plates)
Mix 2.14 grams of drop-out mix with 43.7g MBS653334 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Media Formulation
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases
Amino Acids
Alanine 2.0g
p-Aminobenzoic acid 0.2g
Arginine 2.0g
Asparagine 2.0g
Aspartic acid 2.0g
Cysteine 2.0g
Glutamic acid 2.0g
Glutamine 2.0g
Glycine 2.0g
Histidine 2.0g
Isoleucine 2.0g
Leucine 10.0g
Lysine 2.0g
Methionine 2.0g
Phenylalanine 4.0g
Proline 2.0g
Serine 2.0g
Threonine 2.0g
Tryptophan 2.0g
Tyrosine 4.0g
Valine 2.0g
Nucleotide Bases
*Adenine 0.118g
Inositol 2.0g
Uracil 2.0g

Use 2.14g/liter
100g makes ~50 liters *Final concentration of Adenine is 4.5mg/liter

Product Categories/Family for Drop-out Mix Complete, Adenine Low culture media

Culture Media; Media-Yeast

Other Notes

Small volumes of Drop-out Mix Complete, Adenine Low culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Drop-out Mix Complete, Adenine Low purchase

MBS653334 is a culture media targeting Drop-out Mix Complete, Adenine Low. Also known as Drop-out Mix Complete, Adenine Low (0.118g), High Tyrosine (4g) and Phenylalanine (4g) w/o Yeast Nitrogen Base (Powder).
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