Dulbecco's MEM Culture Media

Off-white, homogeneous, free flowing powder

Cell Culture Grade

Store powdered media at RT.
Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Prepared media should be kept at 4°C and used within a short period of time.

≤ 1EU/ml

Orange, clear, complete.

Light orange, homogenous, free flowing powder.

6.9

Dissolve 15.68g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride•2H20 - 154.520
Cupric Sulfate•5H2O - 0.0013
Ferric Nitrate•9H2O - 0.050
Ferrous Sulfate•7H2O - 0.420
Magnesium Chloride - 28.570
Magnesium Sulfate - 48.830
Potassium Chloride - 311.830
Sodium Bicarbonate - 1200.000
Sodium Chloride - 6999.500
Sodium Phosphate Dibasic - 71.060
Sodium Phosphate Monobasic - 54.350
Zinc Sulfate•7H2O - 0.432

Amino Acids
L-Alanine -
L-Arginine -
L-Asparagine•H2O -
L-Aspartic Acid -
L-Cysteine•HCI•H2O -
L-Cystine•2HCI -
L-Glutamic Acid -
L-Glutamine -
Glycine -
L-Histidine HCl•H2O -
L-Isoleucine -
L-Leucine -
L-Lysine•HCI -
L-Methionine -
L-Phenylalanine -
L-Proline -
L-Serine -
L-Threonine -
L-Tryptophan -
L-Tyrosine•2Na•2H20 -
L-Valine -

Vitamins
Biotin - 0.004
Choline Chloride - 8.980
Vitamin B12 - 0.679
Folic Acid - 2.660
i-Inositol - 0.013
Nicotinamide - 2.018
D-Pantothenic Acid•Ca - 2.120
Pyridoxal HCI - 2.000
Pyridoxine HCI 0.031
Riboflavin - 0.219
Thiamine HCI - 2.170

Other
Thymidine - 0.365
D-Glucose - 3150.000
HEPES Free Acid - 3574.000
Hypoxanthine, Sodium - 2.100
Linoleic Acid - 0.042
Lipoic Acid - 0.105
Phenol Red - 8.305
Putrescine•2HCl - 0.081
Sodium Pyruvate - 55.050
Total: 15.68g/L

Culture Media; Media-Cell Culture; DMEM