BGJb Medium Fitton-Jackson Modification w/L-Glutamine, w/o Phenol Red Culture Media

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/mg

5.7

Colorless, clear, complete

White homogenous, free flowing powder

Dissolve 21.3 g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.5g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Components shown as g/liter:

Inorganic Salts
Magnesium Sulfate 0.09768
Potassium Chloride 0.4
Sodium Acetate 0.050
Sodium Chloride 6.8
Sodium Phosphate Dibasic 0.112
Sodium Phosphate Mono. 0.02434
Amino Acids
L-Alanine 0.25
L-Arginine 0.175
L-Aspartic Acid 0.15
L-Cysteine•HCL•H2O 0.1003
L-Glutamine 0.2
Glycine 0.8
L-Histidine 0.15
L-Isoleucine 0.03
L-Leucine 0.05
L-Lysine•HCl 0.24
L-Methionine 0.05
L-Phenylalanine 0.05
L-Proline 0.4
L-Serine 0.2
L-Threonine 0.075
L-Tryptophan 0.04
L-Tyrosine•2Na•2H2O 0.05766
DL-Valine 0.065
Vitamins
PABA 0.002
Ascorbic Acid, Sodium 0.05
D-Biotin 0.0002
Choline Bitartrate 0.0907
Vitamin B-12 0.00004
Folic Acid 0.0002
myo-Inositol 0.0002
Nicotinic Acid 0.02
D-Pantothenic Acid•Ca 0.0002
Pyridoxal-5-Phosphate 0.0002
Riboflavin 0.0002
Thiamine•HCl 0.004
DL-α-Tocopherol Phosphate•2Na 0.001

Other
Calcium Lactate 0.555
D-Glucose 10
Phenol Red, Sodium absent

Total: 21.3g/liter

Culture Media; Media-Cell Culture