Nutrient Mixture Ham's F-12 Calcium Free w/L-Glutamine, HEPES Culture Media

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Pale orange, homogeneous, free flowing powder.

Pale yellow, clear, complete

5.9

< = 1EU/ml

Dissolve 16.13g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g/L sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Cupric Sulfate•5H2O 0.0000025
Ferrous Sulfate•7H2O 0.000834
Magnesium Chloride•6H2O 0.123
Potassium Chloride 0.2236
Sodium Chloride 7.1
Sodium Phosphate Monobasic 0.14204
Zinc Sulfate•7H2O 0.000863

Amino Acids:
L-Alanine 0.009
L-Arginine•HCl 0.221
L-Asparagine•H2O 0.01501
L-Aspartic Acid 0.0133
L-Cysteine•HCl•H2O 0.035
L-Glutamic Acid 0.0147
L-Glutamine 0.146
Glycine 0.00751
L-Histidine•HCl•H2O 0.02096
L-Isoleucine 0.00394
L-Leucine 0.0131
L-Lysine•HCl 0.0365
L-Methionine 0.00448
L-Phenylalanine 0.00496
L-Proline 0.0345
L-Serine 0.0105
L-Threonine 0.0119
L-Tryptophan 0.00204
L-Tyrosine•2Na•2H2O 0.00778
L-Valine 0.0117

Vitamins:
D-Biotin 0.0000073
Choline Chloride 0.01396
Vitamin B-12 0.00136
Folic Acid 0.00132
myo-Inositol 0.018
Niacinamide 0.000037
D-Pantothenic Acid, Na 0.00048
Pyridoxine•HCl 0.000062
Riboflavin 0.0000376
Thiamine•HCl 0.00034

Other:
Thymidine 0.00073
D-Glucose 1.802
HEPES 5.958
Hypoxanthine 0.00408
Linoleic Acid 0.000084
Thioctic Acid 0.00021
Putrescine•HCl 0.000161
Phenol Red, Sodium 0.0013
Pyruvic Acid, Sodium 0.11

Total: 16.13 g/liter

Culture Media; Media-Cell Culture; Nutrient Mixture Ham's