RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin Culture Media

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.

< 1EU/ml

8.5

White, homogenous, free flowing powder

Colorless, clear, complete.

Cell Culture Grade

Dissolve 10.4g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Inorganic Salts
Calcium Nitrate•4H2O: 0.1 g/liter
Magnesium Sulfate: 0.04884 g/liter
Potassium Chloride: 0.4 g/liter
Sodium Chloride: 6 g/liter
Sodium Phosphate Dibasic: 0.8 g/liter

Amino Acids
L-Arginine: 0.2 g/liter
L-Asparagine (anhydrous): 0.05 g/liter
L-Aspartic Acid: 0.02 g/liter
L-Cystine•2HCl: 0.0652 g/liter
L-Glutamic Acid: 0.02 g/liter
L-Glutamine: 0.3 g/liter
Glycine: 0.01 g/liter
L-Histidine: 0.015 g/liter
Hydroxy-L-proline: 0.02 g/liter
L-Isoleucine: 0.05 g/liter
L-Leucine: 0.05 g/liter
L-Lysine•HCl: 0.04 g/liter
L-Methionine: 0.015 g/liter
L-Phenylalanine: 0.015 g/liter
L-Proline: 0.02 g/liter
L-Serine: 0.03 g/liter
L-Threonine: 0.02 g/liter
L-Tryptophan: 0.005 g/liter
L-Tyrosine•2Na•2H2O: 0.02883 g/liter
L-Valine: 0.02 g/liter

Vitamins
P-Amino Benzoic Acid: 0.001 g/liter
D-Biotin: Absent
Choline Chloride: 0.003 g/liter
Vitamin B-12: 0.000005 g/liter
Folic Acid: 0.001 g/liter
myo-Inositol: 0.035 g/liter
Niacinamide: 0.001 g/liter
D-Pantothenic Acid•Ca: 0.00025 g/liter
Pyridoxine•HCl: 0.001 g/liter
Riboflavin: 0.0002 g/liter
Thiamine•HCl: 0.001 g/liter

Other
D-Glucose: 2 g/liter
Glutathione: 0.001 g/liter
Phenol Red: Absent

Total: 10.4 g/Liter

Hygroscopic. Keep tightly closed and desiccated.

Culture Media; Media-Cell Culture; RPMI