Edinburgh Minimal Media Culture Media

White, homogenous, free flowing powder

Molecular Biology Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Dissolve 32.33g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Base
Potassium Hydrogen Phthalate - 3.00
Sodium Phosphate Dibasic - 2.20
Ammonium Chloride - 5.00
Glucose - 20.00

Salt Stock
Magnesium Chloride•6H2O - 1.05
Calcium Chloride•2H2O - 0.0147
Potassium Chloride - 1.00
Sodium Sulfate - 0.04

Vitamin StockPantothenic Acid•Ca - 0.001
Nicotinic Acid - 0.01
Inositol - 0.01
Biotin - 0.00001

Mineral Stock
Boric Acid - 0.0005
Manganese Sulfate - 0.0004
Zinc Sulfate•7H2O - 0.0004
Ferrous Chloride - 0.0002
Molybdic Acid - 0.00004
Potassium Iodide - 0.0001
Copper Sulfate •5H2O - 0.00004
Citric Acid - 0.001

Total: 32.33g/Liter

The above formulation was derived from the following referenced formula.

Edinburgh Minimal Medium (EMM 2)
3g/L - Potassium hydrogen phthalate (14.7mM)
2.2g/L - Sodium phosphate dibasic (15.5mM)
5g/L - Ammonium chloride (93.5mM)
2% - (w/v) Glucose(111mM)
20ml/L - Salts (50X stock)
1ml/L - Vitamins (1000X stock)
0.1ml/L - Minerals (10,000X stock)

50X Salts
52.5g/L - Magnesium chloride•6H2O (0.26M)
0.735g/L - Calcium chloride•2H2O (4.99mM)
50g/L - Potassium chloride (0.67M)
2g/L - Sodium sulfate (14.1mM)

1000X Vitamins
1g/L - Pantothenic acid (4.20mM)
10g/L - Nicotinic acid (81.2mM)
10g/L - Inositol (55.5mM)
10mg/L - Biotin (40.8uM)

10,000X Minerals

5g/L - Boric Acid (80.9mM)
4g/L - Manganese Sulfate (23.7mM)
4g/L - Zinc Sulfate•7H2O (13.9mM)
2g/L - Ferrous Chloride (7.40mM)
0.4g/L - Molybdic Acid (2.47mM)
1g/L - Potassium Iodide (6.02mM)
0.4g/L - Cupric Sulfate •5H2O (1.60mM)
10g/L - Citric Acid (47.6mM)

5.8

Colorless, clear, complete

Culture Media; Media-Yeast