Medium 199 w/ Earle's, L-Glutamine, w/o Glucose Culture Media

Peach, homogeneous, free-flowing powder

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

≤ 1EU/ml

4.9

Yellow, clear, complete

Dissolve 8.9g in 800-900ml of ddH2O stirring gently until completely solubilized. If required add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Add carbohydrate as required. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts
Calcium Chloride•2H2O: 0.265
Ferric Nitrate•9H2O: 0.00072
Magnesium Sulfate: 0.09767
Potassium Chloride: 0.4
Sodium Acetate: 0.05
Sodium Chloride: 6.8
Sodium Phosphate Monobasic: 0.122
Amino Acids
L-Alanine: 0.05
L-Arginine•HCl: 0.07
L-Aspartic Acid: 0.06
L-Cysteine•HCL•H2O: 0.00011
L-Cystine•2HCl: 0.026
L-Glutamic Acid: 0.1336
L-Glutamine: 0.1
Glycine: 0.05
L-Histidine•HCl•H2O: 0.02188
Hydroxy-L-proline: 0.01
L-Isoleucine: 0.04
L-Leucine: 0.12
L-Lysine•HCl: 0.07
L-Methionine: 0.03
L-Phenylalanine: 0.05
L-Proline: 0.04
L-Serine: 0.05
L-Threonine: 0.06
L-Tryptophan: 0.02
L-Tyrosine•2Na•2H2O: 0.05766
L-Valine: 0.05
Vitamins
P-Aminobenzoic Acid 0.00005
Ascorbic Acid•Na: 0.0000566
D-Biotin: 0.00001
Choline Chloride: 0.0005
Cocarboxylase: 0.00001
Calciferol: 0.0001
myo-Inositol: 0.00005
Menadione, Sodium Bisulfite: 0.000016
Nicotinic Acid: 0.000025
Niacinamide: 0.000025
D-Pantothenic Acid•Ca: 0.00001
Pyridoxal•HCl: 0.000025
Pyridoxine•HCl: 0.000025
Riboflavin: 0.00001
Thiamine•HCl: 0.00001
DL-a-Tocopherol Phosphate•2Na: 0.00001
Retinol Acetate: 0.00014
Other
Adenine Sulfate 0.01
Adenosine Monophosphate: 0.0002385
Adenosine Triphosphate*2Na: 0.001
Cholesterol: 0.0002
2-Deoxy-D-Ribose: 0.0005
D-Glucose: Absent
Glutathione: 0.00005
Guanine•HCl: 0.0003
Hypoxanthine: 0.0003
Phenol Red, Sodium: 0.0213
Tween®80: 0.02
D-Ribose: 0.0005
Thymine: 0.0003
Uracil: 0.0003
Xanthine•Na: 0.000344

Culture Media; Media-Cell Culture