Shields and Sang M3 Insect Medium w/ L-Glutamine Culture Media

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

White, homogeneous, free flowing powder

Light yellow, clear, complete

6.5

Dissolve 39.86 grams in 900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume of 1L. Filter-sterilize using a 0.22 micron membrane. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride: 0.760
Magnesium Sulfate: 2.149
Potassium Bicarbonate: 0.500
Sodium Phosphate Monobasic: 0.880

Amino Acids:
ß-Alanine: 0.250
L-Alanine: 1.500
L-Arginine: 0.500
L-Asparagine: 0.300
L-Aspartic Acid: 0.300
L-Cysteine•HCl: 0.200
L-Glutamic Acid•K: 7.880
L-Glutamic Acid•Na: 6.530
L-Glutamine: 0.600
Glycine: 0.500
L-Histidine: 0.550
L-Isoleucine: 0.250
L-Leucine: 0.400
L-Lysine•HCl: 0.850
L-Methionine: 0.250
L-Phenylalanine: 0.250
L-Proline: 0.400
L-Serine: 0.350
L-Threonine: 0.500
L-Tryptophan: 0.100
L-Tyrosine•2Na: 0.360
L-Valine: 0.400

Vitamins:
Choline Chloride: 0.050
Oxalacetic Acid: 0.250

Other:
D(+)-Glucose: 10.000
BIS-TRIS: 1.050
Yeast Extract: 1.000

Total: 39.860g/L

Culture Media; Media-Insect