Dulbecco's MEM Culture Media

Cell Culture Grade

Store powdered media at RT.
Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Prepared media should be kept at 4 degree C and used within a short period of time.

Light orange, homogeneous, free flowing powder

Pink, clear, complete

7.5

< 1 EU/ml

Dissolve 12.02g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride 2H2O 0.154
Cupric SulfateH2O 0.0000013
Ferric Nitrate 9H2O 0.00005
Ferrous Sulfate 7H2O 0.000417
Magnesium Chloride 6H2O 0.0612
Magnesium Sulfate 0.04884
Potassium Chloride 0.3118
Sodium Chloride 6.996
Sodium Phosphate dibasic 0.07102
Sodium Phosphate monobasic 0.0543
Zinc Sulfate 7H2O 0.000432

Amino Acids:
L-Alanine 0.00445
L-ArginineHCl 0.1475
L-AsparagineH2O 0.0075
L-Aspartic Acid 0.00665
L-Cysteine HCl H2O 0.01756
L-Cystine 2HCl 0.03129
L-Glutamic Acid 0.00735
L-Glutamine 0.365
Glycine 0.01875
L-Histidine HCl H2O 0.03148
L-Isoleucine 0.05447
L-Leucine 0.05905
L-LysineHCl 0.09125
L-Methionine 0.01724
L-Phenylalanine 0.03548
L-Proline 0.01725
L-Serine 0.02625
L-Threonine 0.05345
L-Tryptophan 0.00902
L-Tyrosine 2Na 2H20 0.05579
L-Valine 0.05285

Vitamins:
Biotin absent
Choline Chloride absent
Vitamin B12 absent
Folic Acid absent
i-inositol absent
Nicotinamide absent
D-Pantothenic AcidCa absent
Pyridoxal Hydrochloride absent
Pyridoxine HCl absent
Riboflavin absent
Thiamine HCl absent

Other:
Thymidine 0.000365
D-Glucose 3.15
HypoxanthineNa 0.0021
Linoleic Acid absent
Lipoic Acid absent
Phenol Red, Sodium 0.0081
PutrescineHCl absent
Pyruvic Acid, Sodium 0.055

Total: 12.02g/L

Culture Media; Media-Cell Culture; DMEM