Immunohistochemical analysis of paraffin-embedded mouse brain using D1075-08 DARPP-32 (19A3) Rabbit mAb D1075-08 in the presence of control peptide (left) or DARPP-32 Blocking Peptide (right).

DARPP32 (PPP1R1B) Enzyme

Name: PPP1R1B Enzyme; DARPP32; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS653653
Price: 515 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653653

Peptide is used to block DARPP-32 Rabbit mAb reactivity in immunohistochemistry protocols.DARPP-32 (dopamine and cyclic AMPregulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the… More details

$515 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DARPP32 (PPP1R1B), Enzyme

Full Product Name

DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B) Blocking Peptide

Product Gene Name

PPP1R1B enzyme

Form/Format

Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.

Purity/Purification

Highly Purified
Highly Purified

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

22,963 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (37783177..37792878). Location: 17q12

OMIM

604399

3D Structure

ModBase 3D Structure for Q9UD71

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks DARPP-32 Rabbit mAb signal in immunohistochemistry.

Product Categories/Family for PPP1R1B enzyme

Molecular Biology; MB-Enzymes; Phosphatase

Applications Tested/Suitable for PPP1R1B enzyme

Immunohistochemistry (IHC)

Other Notes

Small volumes of PPP1R1B enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPP1R1B purchase

MBS653653 is an enzyme targeting PPP1R1B. Also known as DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B) Blocking Peptide.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP1R1B. It may not necessarily be applicable to this product.

NCBI GI #

21717548

NCBI GeneID

84152

NCBI Accession #

AAM76683.1

UniProt Primary Accession #

Q9UD71

UniProt Secondary Accession #

Q547V9; Q547W0; Q9H7G1

UniProt Related Accession #

Q9UD71

NCBI Official Full Name

dopamine and cAMP-regulated neuronal phosphoprotein

NCBI Official Synonym Full Names

protein phosphatase 1, regulatory (inhibitor) subunit 1B

NCBI Official Symbol

PPP1R1B

NCBI Official Synonym Symbols

DARPP32; DARPP-32

UniProt Protein Name

Protein phosphatase 1 regulatory subunit 1B

UniProt Synonym Protein Names

DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

Protein Family

Protein phosphatase 1 regulatory

UniProt Gene Name

PPP1R1B

UniProt Synonym Gene Names

DARPP32

UniProt Entry Name

PPR1B_HUMAN

NCBI Protein Information

protein phosphatase 1 regulatory subunit 1B; dopamine and cAMP-regulated neuronal phosphoprotein 32

NCBI Summary for PPP1R1B

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for PPP1R1B

Function: Inhibitor of protein-phosphatase 1.

Subcellular location: Cytoplasm.

Post-translational modification: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.Phosphorylation of Thr-34 is required for activity

By similarity.

Sequence similarities: Belongs to the protein phosphatase inhibitor 1 family.

Research Articles on PPP1R1B

1. PPP1R1B rs12601930 was associated with projection and splitting. rs3764352 was associated with splitting. No significant association was found between rs879606 and defenses. PPP1R1B polymorphisms are involved in immature defenses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PPP1R1B enzyme

Reference Product	PubMed Publications
CDK5 enzyme	>14 publications with PPP1R1B and CDK5

Pathways associated with PPP1R1B enzyme

Products by Pathway	Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
DARPP-32 Events Pathway antibodies	DARPP-32 Events Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Nicotine Activity On Dopaminergic Neurons Pathway antibodies	Nicotine Activity On Dopaminergic Neurons Pathway Diagram

Diseases associated with PPP1R1B enzyme

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>51 publications with PPP1R1B and Nervous System Diseases
Brain Diseases Antibodies	>39 publications with PPP1R1B and Brain Diseases
Movement Disorders Antibodies	>39 publications with PPP1R1B and Movement Disorders
Dyskinesias Antibodies	>29 publications with PPP1R1B and Dyskinesias
Disease Models, Animal Antibodies	>28 publications with PPP1R1B and Disease Models, Animal
Substance-Related Disorders Antibodies	>21 publications with PPP1R1B and Substance-Related Disorders
Cognition Disorders Antibodies	>21 publications with PPP1R1B and Cognition Disorders
Dyskinesia, Drug-Induced Antibodies	>10 publications with PPP1R1B and Dyskinesia, Drug-Induced
Parkinsonian Disorders Antibodies	>10 publications with PPP1R1B and Parkinsonian Disorders
Neurotoxicity Syndromes Antibodies	>10 publications with PPP1R1B and Neurotoxicity Syndromes

Organs/Tissues associated with PPP1R1B enzyme

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>145 publications with PPP1R1B and Brain
Nerve Antibodies	>32 publications with PPP1R1B and Nerve
Stomach Antibodies	>10 publications with PPP1R1B and Stomach
Blood Antibodies	>4 publications with PPP1R1B and Blood
Eye Antibodies	>3 publications with PPP1R1B and Eye
Heart Antibodies	>1 publications with PPP1R1B and Heart
Muscle Antibodies	>1 publications with PPP1R1B and Muscle
Lung Antibodies	>1 publications with PPP1R1B and Lung
Prostate Antibodies	>1 publications with PPP1R1B and Prostate
Placenta Antibodies	>1 publications with PPP1R1B and Placenta