• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Deoxyribonuclease II, Porcine Spleen (DNASE2) Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653670

Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes… More details

$770 / 20K Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Deoxyribonuclease II, Porcine spleen (DNASE2), Enzyme
Full Product Name
Deoxyribonuclease II, Porcine spleen (DNase II)
Product Gene Name
Form/Format
Supplied as a chromatographically purified, dialyzed, lyophilized powder.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
38kD
Chromosome Location
Chromosome: 2; NC_010444.3 (66447514..66450301, complement). Location: chromosome: 2
3D Structure
ModBase 3D Structure for O62855
CAS Number
9003-98-9
Source
Porcine Spleen
Activity
U/mg: 39,912
dw: u/mgP: 46,409
% Protein
86%
Unit Definition
1 unit causes an increase in absorbance at 260nm of 0.001 per minute at 25°C, pH4.6 using highly polymerized DNA as substate.

Product Categories/Family for DNASE2 enzyme

Application Notes for DNASE2 enzyme
DNA Sequencing (acidic pH), DNA Digestion (acidic pH)

Other Notes

Small volumes of DNASE2 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNASE2 purchase

MBS653670 is an enzyme targeting DNASE2. Also known as Deoxyribonuclease II, Porcine spleen (DNase II).

Additional Details

NCBI/Uniprot data below describe general gene information for DNASE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Deoxyribonuclease II
NCBI Official Symbol
DNASE2
UniProt Protein Name
Deoxyribonuclease-2-alpha
UniProt Synonym Protein Names
Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II
Protein Family
UniProt Gene Name
DNASE2
UniProt Synonym Gene Names
DNASE2A; DNL2; DNase II alpha
UniProt Entry Name
DNS2A_PIG
NCBI Protein Information
deoxyribonuclease-2-alpha; acid DNase; DNase II alpha; lysosomal DNase II; deoxyribonuclease II alpha

UniProt Comments for DNASE2

Function: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells

By similarity.

Catalytic activity: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Subcellular location: Lysosome.

Miscellaneous: Not required for the generation of the characteristic DNA fragmentation observed in apoptotic cells, but for the degradation of DNA from dying cells.

Sequence similarities: Belongs to the DNase II family.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000