Phosphodiesterase 2 Enzyme

Name: Enzyme; Phosphodiesterase 2; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS653716
Price: 575 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653716

Spleen exonuclease (Phosphodiesterase II) hydrolyzes 3'-phosphomononucleotides from oligonucleotides containing a 5'-hydroxyl terminus. The enzyme's specificity for the 5'-phosphate end of a polynucleotide chain after conversion to 5'-OH by use of E. coli… More details

$575 / 10 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Phosphodiesterase 2, Enzyme

Full Product Name

Phosphodiesterase 2

Host

Bovine spleen

Form/Format

Supplied as a lyophilized powder from 0.001 M sodium pyrophosphate.

Purity/Purification

Molecular Biology Grade
Purified by chromatography.

Specificity

Native DNA and poly(C) are quite resistant. Bis-(p-nitropnenyl) phosphate is a very poor substrate.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Control No

C15070970

Revision No

110612

Units/mg (dry weight):

1.46u/mg

A280/mg:

0.084

% ADA:

1.2%

Unit Definition:

1 unit increases the absorbance at 260nm by 0.200 in 30 minutes at 37 degree C, pH 6.5, with an RNA Core as substrate.

Certificate of Origin:

The raw animal product (bovine spleen) used in the manufacturing of Phosphodiesterase II is derived from materials originating in North America and collected in USDA approved facilities and inspected to be free of disease. Activity||10 units per vial

Reconstitution:

Reconstitute with sterile ddH20.

Optimum pH:

5.5 using succinate and phosphate buffer and an acid DNase digest; pH 6-7 with 0.1 M acetate buffer.

Product Categories/Family for Phosphodiesterase 2 enzyme

Molecular Biology; MB-Enzymes; Phosphodiesterase

Other Notes

Small volumes of Phosphodiesterase 2 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Phosphodiesterase 2 purchase

MBS653716 is an enzyme targeting Phosphodiesterase 2. Host: Bovine spleen.

Additional Details

NCBI/Uniprot data below describe general gene information for Phosphodiesterase 2. It may not necessarily be applicable to this product.

NCBI GI #

21223851

NCBI Accession #

NP_629630.1

GenBank Nucleotide #

NC_003888.3

NCBI Official Full Name

phosphodiesterase

Protein Family

Sphingomyelin phosphodiesterase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.