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Secretase, Alpha FRET Peptide, MCA-HQKLVFFA Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653725

Fluorescent resonance energy transfer (FRET) peptide for detecting a-secretase. Labeled with methoxycoumarin acetic acid (MCA) and dinitrophenyl (DNP). More details

$685 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Secretase, alpha FRET Peptide, MCA-HQKLVFFA, Enzyme
Full Product Name
Secretase, alpha FRET Peptide, MCA-HQKLVFFA (K-DNP)
Form/Format
Supplied as a lyophilized powder.
Purity/Purification
Highly Purified
~90% by HPLC and MS analysis
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Stable for 12 months at -20 degree C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
1499
Sequence (linear)
MCA-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-(Lys-DNP)-OH
Amino Acid Analysis
Confirms expected sequence
Peptide Content: 80.0 ± 4%
Solubility
H2O

Testing Data of Secretase, alpha FRET Peptide, MCA-HQKLVFFA enzyme

Absorption|This is a graph of the molar extinction coefficient of 7-Methoxycoumarin-4-acetic acid dissolved in methanol. It was measured by R. A. Fuh on summer, 95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. 7-Methoxycoumarin-4-acetic acid has a molar extinction coefficient of 11,820
Enzyme – Cat# MBS653725

Testing Data of Secretase, alpha FRET Peptide, MCA-HQKLVFFA enzyme

R. Farinotti, P.|This is the fluorescence emission spectrum of 7-Methoxycoumarin-4-acetic acid dissolved in methanol. The spectrum was taken by R. A. Fuh on summer, 95 using an excitation wavelength of 275 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.18 [R. Farinotti, P. Siard, J. Bourson, S. Kirkiacharian, B. Valeur, and G. Mahuzier, "4-Bromomethyl-6,7-dimethoxycoumarin as a fluorescent label for carboxylic acids in chromatographic detection," J. Chromatography, 269, 81-90, 1983].
Enzyme, Testing Data – Cat# MBS653725

Other Notes

Small volumes of Secretase, alpha FRET Peptide, MCA-HQKLVFFA enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Secretase, alpha FRET Peptide, MCA-HQKLVFFA purchase

MBS653725 is an enzyme targeting Secretase, alpha FRET Peptide, MCA-HQKLVFFA. Also known as Secretase, alpha FRET Peptide, MCA-HQKLVFFA (K-DNP).
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