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Collagenase, Type III, C. Histolyticum Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653735

Description: Collagenase belongs to the peptidase family M10B. Type III, containing low proteolytic activity. It is usually used for mammary and fetal cells. Type III cleaves gelatin Type I and collagen Types IV, V, VII, C, cleaving the collagen-like… More details

$460 / 100 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Collagenase, Type III, C. histolyticum, Enzyme
Full Product Name
Collagenase, Type III, C. histolyticum
Host
Clostridium histolyticum
Form/Format
Supplied as a dialyzed, lyophilized powder.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number
9001-12-1
Unit Definition
1 unit releases 1 µmole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.
Units/mg (dw)
270
Units/mg Caseinase (dw)
74
Units/mg Clostripain(dw)
0.68
Units/mg Tryptic (dw)
0.03
Absorbance mg/ml @ 280nm
0.99
Suitable for use in mammary primary cell isolation.Collagenase is prepared to contain lower proteolytic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity.

Other Notes

Small volumes of Collagenase, Type III, C. histolyticum enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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MBS653735 is an enzyme targeting Collagenase, Type III, C. histolyticum. Host: Clostridium histolyticum.
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