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Alanine Aminotransferase (GPT) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS173090

MBS173090 is an active protein targeting GPT. Also known as Glutamate Pyruvate Transaminase (GPT), Alanine Transaminase (ALT). Host: Porcine Heart. More details

$235 / 1000 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alanine Aminotransferase (GPT), Active Protein
Full Product Name
Glutamate Pyruvate Transaminase (GPT), Alanine Transaminase (ALT)
Product Gene Name
Product Synonym Gene Name
AAT1; GPT1
Product Synonym Names
Alanine Transaminase, ALT, GPT
Host
Porcine Heart
Form/Format
Lyophilized from tris-chloride buffer, pH 7.0 with sodium chloride, EDTA, DTT, alpha-ketoglurarte, and pyridoxal-5-phosphate
Preparation and Storage
Store at -20°C
Protect from moisture
3D Structure
ModBase 3D Structure for P13191
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Appearance
Yellow to yellow-green powder
Solubility
Clear, colorless to yellow-green solution (1 mg/mL saline)
Activity
200 U/mg (Dimension® Clinical Chemistry System)
Protein
0.92 mg protein/mg (A280)
0.59 mg protein/mg (Coomassie)
Specific Activity
220 U/mg protein (A280)
340 U/mg protein (Coomassie)
Unit Definition
One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.
Contaminants
CPK: < 0.1%
GLDH: < 0.01%
LDH: < 0.1%
SGOT/AST: < 0.5%
Protease: < 0.02 U/mg (< 0.001 O.D./8 min.)
Ammonia
< 0.5 umole/mg
Advice
Reconstitute into provided container and centrifuge to ensure full recovery.
Handling Information
Aliquot and freeze at -20°C after reconstitution.
Handle as a potentially hazardous substance.
CAS Number
9000-86-6
E.C Number
2.6.1.2

Other Notes

Small volumes of GPT active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
Alanine aminotransferase 1
UniProt Protein Name
Alanine aminotransferase 1
UniProt Synonym Protein Names
Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
UniProt Gene Name
GPT
UniProt Synonym Gene Names
AAT1; GPT1; ALT1; GPT 1
UniProt Entry Name
ALAT1_PIG

UniProt Comments for GPT

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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