Angiotensin Converting Enzyme, Rat (ACE) Enzyme

Name: ACE Enzyme; Angiotensin Converting Enzyme, Rat; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS653765
Price: 1230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653765

ACE (kininase II, EC 3.4.15.1) is a zinc dipeptidyl carboxypeptidase. ACE catalyses both angiotensin I transformation to angiotensis II (a potent vasopressor agent) and degradation of bradikinin, a vaso-depressor. ACE appears to play a key role in regulating… More details

$1230 / 1 Unit +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Angiotensin Converting Enzyme, Rat (Ace), Enzyme

Full Product Name

Angiotensin Converting Enzyme, Rat (ACE)

Product Gene Name

Ace enzyme

Host

Rat lung

Form/Format

Supplied as a liquid in 100mM PBS, 150mM sodium chloride, pH 7.4 with 0.2mM CHAPS. No preservatives added.

Purity/Purification

Highly purified (> 95% by SDS-PAGE).

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

150,908 Da

Chromosome Location

Chromosome: 10; NC_005109.3 (93922062..93965127). Location: 10q32.1

3D Structure

ModBase 3D Structure for P47820

Activity

~7mU/ ug of protein
(One unit will produce 1uMole of hippuric acid or His-Leu from Z-Phe-His-Leu per minute in 0.1M phosphate buffer, 30 mM sodium chloride, at pH 8.3 at 37 degree C).

Product Categories/Family for Ace enzyme

Molecular Biology; MB-Enzymes; Angiotensin Converting (ACE)

Applications Tested/Suitable for Ace enzyme

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for Ace enzyme

Suitable for use as a reference in SDS-PAGE and Western blotting (together with anti-ACE monoclonal), as a reference antigen for immunoprecipitation, and as a source of pure ACE for different enzymatic studies of ACE.
Dilution: Western Blot: 500ng/lane if gel is developed with anti-ACE antibody. Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of Ace enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ace purchase

MBS653765 is an enzyme targeting Ace. Also known as Angiotensin Converting Enzyme, Rat (ACE). Host: Rat lung.

Additional Details

NCBI/Uniprot data below describe general gene information for Ace. It may not necessarily be applicable to this product.

NCBI GI #

6978757

NCBI GeneID

24310

NCBI Accession #

NP_036676.1

UniProt Primary Accession #

P47820

UniProt Secondary Accession #

Q7TMC6; Q8CFN1; Q9EQM9

UniProt Related Accession #

P47820

GenBank Nucleotide #

NM_012544.1

NCBI Official Full Name

angiotensin-converting enzyme

NCBI Official Synonym Full Names

angiotensin I converting enzyme

NCBI Official Symbol

Ace

NCBI Official Synonym Symbols

Dcp1; StsRR92

UniProt Protein Name

Angiotensin-converting enzyme

UniProt Synonym Protein Names

Dipeptidyl carboxypeptidase I; Kininase II

Protein Family

Accessory cholera enterotoxin

UniProt Gene Name

Ace

UniProt Synonym Gene Names

Dcp1; ACE

UniProt Entry Name

ACE_RAT

NCBI Protein Information

angiotensin-converting enzyme; kininase II; dipeptidyl carboxypeptidase I; angiotensin 1 converting enzyme 1; angiotensin I converting enzyme 1; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; Dipeptidyl carboxypeptidase 1 (Angiotensin I-converting enzyme); angiotensin I-converting enzyme (Dipeptidyl carboxypeptidase 1)

NCBI Summary for Ace

catalyzes the conversion of angiotensin I to angiotensin II; plays a role in regulation of blood pressure [RGD, Feb 2006]

UniProt Comments for Ace

Function: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm

By similarity.

Catalytic activity: Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.

Cofactor: Binds 2 zinc ions per subunit. Isoform Testis-specific only binds 1 zinc ion per subunit

By similarity.Binds 3 chloride ions per subunit

By similarity.

Subcellular location: Angiotensin-converting enzyme, soluble form: Secreted

By similarity. Cell membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Testis-specific isoform is expressed in spermatocytes, adult testis. Also expressed in brain, kidney, lung, skeletal muscle and heart. Ref.3

Induction: Up-regulated after myocardial infarction. Ref.6

Post-translational modification: Phosphorylated by CK2 on Ser-1306; which allows membrane retention

By similarity.

Sequence similarities: Belongs to the peptidase M2 family.

Research Articles on Ace

1. Increased expression of pulmonary ACE contributes to elevated circulating angiotensin II in gestational protein restriction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Ace enzyme

Products by Pathway	Pathway Diagram
ACE Inhibitor Pathway antibodies	ACE Inhibitor Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies	Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Peptide Hormone Metabolism Pathway antibodies	Peptide Hormone Metabolism Pathway Diagram
Renin - Angiotensin System Pathway antibodies	Renin - Angiotensin System Pathway Diagram
Renin-angiotensin System Pathway antibodies	Renin-angiotensin System Pathway Diagram

Diseases associated with Ace enzyme

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>13366 publications with Ace and Cardiovascular Diseases
Hypertension Antibodies	>8591 publications with Ace and Hypertension
Heart Failure Antibodies	>4528 publications with Ace and Heart Failure
Kidney Diseases Antibodies	>3368 publications with Ace and Kidney Diseases
Myocardial Infarction Antibodies	>2508 publications with Ace and Myocardial Infarction
Coronary Artery Disease Antibodies	>2157 publications with Ace and Coronary Artery Disease
Coronary Disease Antibodies	>2141 publications with Ace and Coronary Disease
Stroke Antibodies	>1363 publications with Ace and Stroke
Renal Insufficiency Antibodies	>1280 publications with Ace and Renal Insufficiency
Respiratory Tract Diseases Antibodies	>1240 publications with Ace and Respiratory Tract Diseases

Organs/Tissues associated with Ace enzyme

Organ/Tissue Name	Pubmed Publications
Artery Antibodies	>3460 publications with Ace and Artery
Lung Antibodies	>1546 publications with Ace and Lung
Brain Antibodies	>1246 publications with Ace and Brain
Eye Antibodies	>233 publications with Ace and Eye
Testis Antibodies	>223 publications with Ace and Testis
Connective Tissue Antibodies	>183 publications with Ace and Connective Tissue
Embryonic Tissue Antibodies	>138 publications with Ace and Embryonic Tissue
Small Intestine Antibodies	>120 publications with Ace and Small Intestine
Ovary Antibodies	>114 publications with Ace and Ovary
Colon Antibodies	>99 publications with Ace and Colon