• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

T4 Endonuclease V Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653788

T4 Pyrimidine DNA Glycosylase Bifunctional DNA Glycosylase with DNA N-glycosylase and AP Lyase Activities. Useful in studies of UV damage to DNA and its repair, including DNA damage in single cells. Detection of differential UV damage repair of transcribed… More details

$430 / 2000 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
T4 Endonuclease V, Enzyme
Full Product Name
T4 Endonuclease V (T4 PDG)
Host
gene denV in E. coli
Concentration
10 U/uL (lot specific)
Preparation and Storage
Stable for one year at -20ºC.
Reagents Supplied
The following reagents are supplied with this product:
Component Name: T4 PDG (T4 Endonuclease V)
Store at (°C): -20
Amount: 1 x 0.2ml
Concentration: 10,000U/ml

Component Name: T4 PDG Reaction Buffer
Store at (°C): -20
Amount: 1 x 1.5ml
Concentration: 10X

Component Name: BSA
Store at (°C): -20
Amount: 1x0.25ml
Concentration: 10mg/ml
Activity
10u/ul
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5ug of UV irradiated supercoiled pUC19 DNA to > 95% nicked plasmid in a total reaction volume of 20ul in 30 minutes at 37°C. Nicking is assessed by agarose gel electrophoresis. Irradiated plasmid contains an average of 3-5 pyrimidine dimers.
Reaction Conditions
1X T4 PDG Reaction Buffer Supplement with 100X Purified BSA Incubate at 37°C
1X T4 PDG Reaction Buffer
100mM NaCl
1mM DTT
1mM EDTA
25mM Na2HPO4 (pH 7.2 @ 25°C)
Storage Buffer
10mM Tris-HCl 250mM NaCl 1mM DTT 0.1mM EDTA 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C
Heat Inactivation
No
Unit Assay Conditions
1X T4 PDG Reaction Buffer containing 0.5ug of UV irradiated supercoiled pUC19 DNA, supplemented with 100 µg/ml BSA in a 20ul reaction.
Quality Control
T4 Endonuclease V is free of detectable nonspecific exo-and endonuclease activities and RNase activity.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for T4 Endonuclease V enzyme

Molecular Biology; MB-Enzymes; Endonuclease

Testing Data of T4 Endonuclease V enzyme

Enzyme – Cat# MBS653788

Testing Data of T4 Endonuclease V enzyme

Figure 1. Mechanism of pyrimidine dimer cleavage by T4 Endonuclease V.| (Adapted with permission from Friedburg, E.C. et al. (1995) DNA Repair and Mutagenesis, ASM Press.)
Enzyme, Testing Data – Cat# MBS653788

Other Notes

Small volumes of T4 Endonuclease V enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for T4 Endonuclease V purchase

MBS653788 is an enzyme targeting T4 Endonuclease V. Also known as T4 Endonuclease V (T4 PDG). Host: gene denV in E. coli.

Additional Details

NCBI/Uniprot data below describe general gene information for T4 Endonuclease V. It may not necessarily be applicable to this product.

NCBI GI #
533269
NCBI Accession #
AAA72721.1
NCBI Official Full Name
T4 endonuclease V
Protein Family
Putative NmeDIP very short patch repair endonuclease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000