Structure of MBS653872
RNase A, Pancreatic Ribonuclease, Rib 1; Ribonuclease A Family 1; Ribonuclease Pancreatic; Ribonuclease RNase A Family 1; Ribonuclease RNase A Family 1 Pancreatic; RNAseA; RNase 1; RNASE1; RNS1

Ribonuclease A, Bovine Pancreas (RNASE1) Enzyme

Name: RNASE1 Enzyme; Ribonuclease A, Bovine Pancreas; Bovine; MyBioSource
Brand: MyBioSource
SKU: MBS653872
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653872

Ribonuclease A is used to isolate DNA for RNA-free DNA. Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3’-Phosphate group of a pyrimidine nucleotide and the 5’-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic… More details

$220 / 100 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ribonuclease A, Bovine Pancreas (RNASE1), Enzyme

Full Product Name

Ribonuclease A, Bovine Pancreas (RNase A)

Product Gene Name

RNASE1 enzyme

Product Synonym Names

E.C.3.1.27.5; Ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; Ribonuclease 1

Form/Format

Off-white powder

Preparation and Storage

Best stored desiccated at -20 degree C or below. Stable for 6 months after receipt. However, the enzyme is also stored as a refrigerated dry powder or frozen in aqueous solution.

Molecular Weight

13.7kD

Chromosome Location

Chromosome: 14; NC_000014.8 (21269515..21271036, complement). Location: 14q11.2

OMIM

180440

3D Structure

ModBase 3D Structure for P07998

Source

Bovine pancreas

CAS Number

9001-99-4

Activity

78 u/mg

Solubility

Soluble at 10mg/ml in analytical grade water. Colorless, clear, complete.

Unit Definition

One unit will cause the hydrolysis of RNA at that rate where the velocity constant k equals unity (Kunitz units) at 25ºC and pH 5.0.

Nomenclature

E;C.3.1.27.5; Ribonucleate 3-pyrimidino-oligonucleotidohydralase; Ribonuclease 1

Note

Hygroscopic. Keep tightly closed and desiccated

Product Categories/Family for RNASE1 enzyme

Cloning; Cloning-Enzymes

Other Notes

Small volumes of RNASE1 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNASE1 purchase

MBS653872 is an enzyme targeting RNASE1. Also known as Ribonuclease A, Bovine Pancreas (RNase A).

Additional Details

NCBI/Uniprot data below describe general gene information for RNASE1. It may not necessarily be applicable to this product.

NCBI GI #

38201686

NCBI GeneID

6035

NCBI Accession #

NP_937878.1

UniProt Primary Accession #

P07998

UniProt Secondary Accession #

Q16830; Q16869; Q1KHR2; Q6ICS5; Q9UCB4; Q9UCB5; B2R589; D3DS06

UniProt Related Accession #

P07998

GenBank Nucleotide #

NM_198235.2

NCBI Official Full Name

ribonuclease pancreatic

NCBI Official Synonym Full Names

ribonuclease, RNase A family, 1 (pancreatic)

NCBI Official Symbol

RNASE1

NCBI Official Synonym Symbols

RIB1; RNS1

UniProt Protein Name

Ribonuclease pancreatic

UniProt Synonym Protein Names

HP-RNase; RIB-1; RNase UpI-1; Ribonuclease 1; RNase 1; Ribonuclease A

Protein Family

Ribonuclease pancreatic

UniProt Gene Name

RNASE1

UniProt Synonym Gene Names

RIB1; RNS1; RNase 1; RNase A

UniProt Entry Name

RNAS1_HUMAN

NCBI Protein Information

ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A

NCBI Summary for RNASE1

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

UniProt Comments for RNASE1

Function: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA. Ref.19

Catalytic activity: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Subunit structure: Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein. Ref.19

Subcellular location: Secreted.

Tissue specificity: Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion).

Post-translational modification: N-linked glycans are of complex type.

Sequence similarities: Belongs to the pancreatic ribonuclease family.

Research Articles on RNASE1

1. The reduced conformational flexibility of eosinophil cationic protein can be dynamically and functionally reproduced in the RNase A scaffold.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with RNASE1 enzyme

Disease Name	Pubmed Publications
Pancreatic Neoplasms Antibodies	>3 publications with RNASE1 and Pancreatic Neoplasms
Disease Models, Animal Antibodies	>3 publications with RNASE1 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>2 publications with RNASE1 and Neoplasms, Experimental
Mammary Neoplasms, Experimental Antibodies	>1 publications with RNASE1 and Mammary Neoplasms, Experimental
Breast Neoplasms Antibodies	>1 publications with RNASE1 and Breast Neoplasms
Edema Antibodies	>1 publications with RNASE1 and Edema
Necrosis Antibodies	>1 publications with RNASE1 and Necrosis
Inflammation Antibodies	>1 publications with RNASE1 and Inflammation

Organs/Tissues associated with RNASE1 enzyme

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>3 publications with RNASE1 and Vascular
Liver Antibodies	>1 publications with RNASE1 and Liver
Muscle Antibodies	>1 publications with RNASE1 and Muscle
Pancreas Antibodies	>1 publications with RNASE1 and Pancreas
Mammary Gland Antibodies	>1 publications with RNASE1 and Mammary Gland
Stomach Antibodies	>1 publications with RNASE1 and Stomach
Lung Antibodies	>1 publications with RNASE1 and Lung