Tyrosine Decarboxylase, Holoenzyme Enzyme

Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary coenzyme. By utilizing the apoenzyme (TYDAPO), prepared from cells grown on a vitamin B6 deficient medium, pyridoxal phosphate may be determined. Decarboxylation of radio-labeled tyrosine has permitted the determination of much smaller amounts of pyridoxal phosphate.

Acetone-dried cells were used as the source in a relatively simple extraction and purification of the apoenzyme. The holoenzyme may be used to determine tyrosine, phenylalanine and dihydroxyphenylalanine either manometrically or colorimetrically. Tashian (1957) has reported a determination of tyrosine in urine based on this principle. Plasma phenylalanine can also be determined using this enzyme. Poisner (1960) determined serum phenylalanine levels in schizophrenics using Hsia's method and obtained a significant elevation over normals.

Small volumes of Tyrosine Decarboxylase, Holoenzyme enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS653815 is an enzyme targeting Tyrosine Decarboxylase, Holoenzyme. Host: Streptococcus faecalis (6783).