RNA Ligase T4, 10-20u/Ul (RNA) Enzyme

Purified from an E.coli strain carrying a T4 RNA ligase overproducing plasmid. Catalyses the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of single-stranded RNA or DNA. The minimal substrate is a nucleoside 3', 5'-diphosphate.

ApplicationsRNA 3'-end labelling (1)Joining RNA to RNA (2)Circularization of oligonucleotides (3)Joining synthetic oligodeoxyribonucleotides (4)Specific modifications of tRNAs (5)Oligodeoxyribonucleotide ligation to single-stranded cDNAs (6)Generation of composite primers to PCR (7)

Small volumes of RNA Ligase T4, 10-20u/ul enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS653841 is an enzyme targeting RNA Ligase T4, 10-20u/ul.