• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Mung Bean Nuclease Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653860

Mung Bean Nuclease belongs to a class of enzymes that demonstrate a preference for single-stranded nucleic acids, lack sugar specificity and hydrolyze single-stranded substrates to products with 5'-phosphoryl and 3'-hydroxyl termini. Mung Bean Nuclease… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Mung Bean Nuclease, Enzyme
Full Product Name
Mung Bean Nuclease
Form/Format
Supplied as a liquid in 0.01M Tris-HCl, pH 7.5, 0.05M sodium chloride, 50% glycerol, 0.01% Triton X-100.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
39kD
Quality Control
Contaminant Endonuclease/Nickase Activity: To confirm the absence of contaminating endonuclease/nickase activity, 1ug of lambda DNA/ Hind III markers is incubated with Mung Bean Nuclease for 10 minutes at RT. The markers are then separated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Markers that have been incubated with 60u of enzyme will remain as intact bands with a minimal amount of smearing.
Unit Definition
One unit is defined as the amount of enzyme required to produce 1ug of acid-soluble nucleotides per minute at 37 degree C in 30mM sodium acetate, pH 5.0, 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA, 5% glycerol.
Application Notes for Mung Bean Nuclease enzyme
Suitable for use in Transcript Mapping, Production of blunt ends for cloning, Flushing staggered ends and Separation of cDNA strands after synthesis with reverse transcriptase and DNA polymerase I.

Other Notes

Small volumes of Mung Bean Nuclease enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mung Bean Nuclease purchase

MBS653860 is an enzyme targeting Mung Bean Nuclease.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000